Immunohistochemical examination of myogenesis and expression pattern of myogenic regulatory proteins (myogenin and myf-3) in pigs
نویسندگان
چکیده
An experiment was conducted to immunohistochemically investigate myogenesis and the expression pattern of two myogenic regulatory proteins, myf-3 and myogenin, during formation of secondary fibres in pigs. Muscle fibres develop as two distinct populations in the foetal pig. Our data in foetal pigs suggest that formation of secondary fibres occurs from about day 60 to 80 of gestation and that myogenesis is concluded at approximately day 80 of gestation. The frequency of nuclei expressing myf-3 did not change, while that of nuclei expressing myogenin decreased from day 60 to 100 of gestation. Thus, myf-3 and myogenin appear to exhibit different expression patterns during myogenesis. The significance of decline in the frequency of nuclei expressing myogenin during secondary muscle fibre formation is not clear. During postnatal muscle growth no clear relationships between the expression pattern of myf-3 and myogenin with different fibre types were established. 2000 Elsevier Science B.V. All rights reserved.
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